Agarose-based system for separation of short tandem repeat loci.

Genetic Variation of Informative Short Tandem Repeat (STR) Loci in an Iranian Population

In the present study, genotyping of six short tandem repeat (STR) loci including CSF1PO, D16S539, F13A01, F13B, LPL and HPRTB was performed on genomic DNA from 127 unrelated individuals from the Iranian province of Isfahan. The results indicated that the allele and genotype distributions were in accordance with Hardy-Weinberg expectations. The observed heterozygosity (Ho), expected heterozygosi...

Improved electrophoretic separation of polymorphic short tandem repeats in agarose gels using bis-benzimide.

Genetic analysis of two STR loci (VWA and TPOX) in the Iranian province of Khuzestan

Objective(s): Short tandem repeat (STR) loci are the most informative DNA genetic markers for attempting to individualize biological material for application in paternity and forensic cases. Materials and Methods: Blood samples were collected and the total genomic DNA was extracted. The DNA samples were used for genotyping VWA and TPOX STR loci using PCR and polyacrylamide gel electrophoresis. ...

Genotyping of Five Polymorphic STR Loci in Iranian Province of Isfahan

Genotyping for five short tandem repeat (STR) loci HUMvWA, HUMFES, HUMTPO, HUMTH01 and D3S1359 was done in 220 unrelated individuals from the population of Isfahan province of IR Iran. The loci were genotyped using the polymerase chain reaction (PCR) followed by polyacrylamide gel electrophoresis (PAGE) and silver staining. The data demonstrated that the STR markers were all found informative i...

Multiple polymorphic loci for molecular typing of strains of Mycobacterium leprae.

The need for molecular tools for the differentiation of isolates of Mycobacterium leprae, the organism that causes leprosy, is urgent in view of the continuing high levels of new case detection, despite years of aggressive chemotherapy and the consequent reduction in the prevalence of leprosy. The slow onset of leprosy and the reliance on physical examination for detection of disease have restr...